Journal: bioRxiv

Article Title: Overcoming efflux of fluorescent probes for actin imaging in living cells

doi: 10.1101/2020.02.17.951525

Figure Lengend Snippet: Confocal and nanoscopy images of living cells and tissues stained with the new actin probes. (a) Comparison of confocal and STED images of human fibroblasts stained with 6-610CP-JAS. (b) Human melanocytes co-stained with 6-580CP-JAS and 5-SiR-Hoechst. (c) live-cell imaging of body wall muscle of dissected D. melanogaster larva costained with 6-580CP-JAS and 6-SiR-CTX. (d) Max intensity projection of a frog erythrocyte stained with 6-610CP-JAS. (e) Rat primary neuron culture co-stained with 6-610CP-JAS and neurofascin (AlexaFluor 488). Scale bars 10 μm (a-d), 5 μm (e).

Article Snippet: Normal neonatal human melanocytes (MatTek Corporation, Cat. NHM-CRY-NEO) were cultured in Normal human melanocyte cells growth medium (MatTek Corporation, Cat. NHM-GM) in a humidified 5% CO2 incubator at 37 °C.

Techniques: Staining, Comparison, Live Cell Imaging

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: LncRNA LNCOC1 is Upregulated in Melanoma and Serves as a Potential Regulatory Target of miR-124 to Suppress Cancer Cell Invasion and Migration

doi: 10.2147/CCID.S359786

Figure Lengend Snippet: The expression levels of LNCOC1 were increased in melanoma cell lines and enhanced cell proliferation of primary human melanocytes. Expression of LNCOC1 and miR-124 in primary human melanocytes and melanoma cell lines were detected by RT-qPCR. The expression levels of LNCOC1 were much higher in SK-MEL-3 and A375 than that in primary melanocytes ( A and B ). Overexpressed LNCOC1 could promote the proliferation of primary melanocytes. As a suppressive factor in cancer, miR-124 had an inhibitory effect on melanocytes proliferation ( C ). * p < 0.05.

Article Snippet: Primary neonatal normal human epidermal melanocytes were obtained from Sangon Biotech (Shanghai, China).

Techniques: Expressing, Quantitative RT-PCR

Journal: Clinical, Cosmetic and Investigational Dermatology

Article Title: LncRNA LNCOC1 is Upregulated in Melanoma and Serves as a Potential Regulatory Target of miR-124 to Suppress Cancer Cell Invasion and Migration

doi: 10.2147/CCID.S359786

Figure Lengend Snippet: The scheme of mechanism of the effect of LNCOC1 and miR-124 on melanoma. In MM patients, LNCOC1 displayed an elevated level in their tumor tissues. Also, a decrease in miR-124 was found in those tumor samples, which might contribute to the poor survival of MM patients. On the other hand, overexpression of miR-124 could downregulate LNCOC1 expression, suggesting LNCOC1 is an inhibitory target of miR-124 to suppress the invasion and migration of melanoma cells. In addition, LNCOC1 reduction and miR-124 elevation inhibited primary human melanocyte proliferation to play protective roles in the progression of melanoma.

Article Snippet: Primary neonatal normal human epidermal melanocytes were obtained from Sangon Biotech (Shanghai, China).

Techniques: Over Expression, Expressing, Migration